Dominko, Kristina; Talajić, Antea; Radic, Martina; Vidaček, Nikolina Škrobot; Vlahoviček, Kristian; Bosnar, Maja Herak; Ćetković, Helena (2023). Transfection of Sponge Cells and Intracellular Localization of Cancer-Related MYC, RRAS2, and DRG1 Proteins. Marine drugs, 21(2) MDPI 10.3390/md21020119
|
Text
marinedrugs-21-00119-v2.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (5MB) | Preview |
The determination of the protein's intracellular localization is essential for understanding its biological function. Protein localization studies are mainly performed on primary and secondary vertebrate cell lines for which most protocols have been optimized. In spite of experimental difficulties, studies on invertebrate cells, including basal Metazoa, have greatly advanced. In recent years, the interest in studying human diseases from an evolutionary perspective has significantly increased. Sponges, placed at the base of the animal tree, are simple animals without true tissues and organs but with a complex genome containing many genes whose human homologs have been implicated in human diseases, including cancer. Therefore, sponges are an innovative model for elucidating the fundamental role of the proteins involved in cancer. In this study, we overexpressed human cancer-related proteins and their sponge homologs in human cancer cells, human fibroblasts, and sponge cells. We demonstrated that human and sponge MYC proteins localize in the nucleus, the RRAS2 in the plasma membrane, the membranes of the endolysosomal vesicles, and the DRG1 in the cell's cytosol. Despite the very low transfection efficiency of sponge cells, we observed an identical localization of human proteins and their sponge homologs, indicating their similar cellular functions.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Urologie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Urologie |
UniBE Contributor: |
Radic, Martina |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1660-3397 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
03 Mar 2023 10:49 |
Last Modified: |
03 Mar 2023 23:28 |
Publisher DOI: |
10.3390/md21020119 |
PubMed ID: |
36827160 |
Uncontrolled Keywords: |
DRG1 MYC Porifera RRAS2 intracellular localization primary sponge cells transfection |
BORIS DOI: |
10.48350/179232 |
URI: |
https://boris.unibe.ch/id/eprint/179232 |
Actions (login required)
 |
Edit item |