Urinary soluble HLA-DR is a potential biomarker for acute renal transplant rejection

Ting, Yi Tian; Coates, P Toby; Marti, Hans-Peter; Dunn, Amy C; Parker, Richard M; Pickering, John W; Jack, Ralph W; Kemp, Roslyn A; Walker, Robert J; McLellan, Alexander D (2010). Urinary soluble HLA-DR is a potential biomarker for acute renal transplant rejection. Transplantation, 89(9), pp. 1071-8. Hagerstown, Md.: Lippincott Williams & Wilkins 10.1097/TP.0b013e3181d15492

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BACKGROUND.: Urine is a potentially rich source of biomarkers for monitoring kidney dysfunction. In this study, we have investigated the potential of soluble human leukocyte antigen (sHLA)-DR in the urine for noninvasive monitoring of renal transplant patients. METHODS.: Urinary soluble HLA-DR levels were measured by sandwich enzyme-linked immunosorbent assay in 103 patients with renal diseases or after renal transplantation. sHLA-DR in urine was characterized by Western blotting and mass spectrometry. RESULTS.: Acute graft rejection was associated with a significantly elevated level of urinary sHLA-DR (P<0.0001), compared with recipients with stable graft function or healthy individuals. A receiver operating characteristic curve analysis showed the area under the curve to be 0.88 (P<0.001). At a selected threshold, the sensitivity was 80% and specificity was 98% for detection of acute renal transplant rejection. sHLA-DR was not exosomally associated and was of lower molecular weight compared with the HLA-DR expressed as heterodimer on the plasma membrane of antigen-presenting cells. CONCLUSIONS.: sHLA-DR excreted into urine is a promising indicator of renal transplant rejection.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension

UniBE Contributor:

Marti, Hans-Peter

ISSN:

0041-1337

Publisher:

Lippincott Williams & Wilkins

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:11

Last Modified:

04 May 2014 23:05

Publisher DOI:

10.1097/TP.0b013e3181d15492

PubMed ID:

20098353

Web of Science ID:

000277243700004

URI:

https://boris.unibe.ch/id/eprint/1795 (FactScience: 203821)

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