Solberg, Therese; Mason, Victor; Wang, Chundi; Nowacki, Mariusz (2023). Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium. Cell reports, 42(3), p. 112213. Cell Press 10.1016/j.celrep.2023.112213
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The clearance of untranslated mRNAs by Argonaute proteins is essential for embryonic development in metazoans. However, it is currently unknown whether similar processes exist in unicellular eukaryotes. The ciliate Paramecium tetraurelia harbors a vast array of PIWI-clade Argonautes involved in various small RNA (sRNA) pathways, many of which have not yet been investigated. Here, we investigate the function of a PIWI protein, Ptiwi08, whose expression is limited to a narrow time window during development, concomitant with the start of zygotic transcription. We show that Ptiwi08 acts in an endogenous small interfering RNA (endo-siRNA) pathway involved in the clearance of untranslated mRNAs. These endo-siRNAs are found in clusters that are strictly antisense to their target mRNAs and are a subset of siRNA-producing clusters (SRCs). Furthermore, the endo-siRNAs are 2'-O-methylated by Hen1 and require Dcr1 for their biogenesis. Our findings suggest that sRNA-mediated developmental mRNA clearance extends beyond metazoans and may be a more widespread mechanism than previously anticipated.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology |
UniBE Contributor: |
Solberg, Therese, Mason, Victor Christian, Wang, Chundi, Nowacki, Mariusz |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
2211-1247 |
Publisher: |
Cell Press |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
06 Mar 2023 09:10 |
Last Modified: |
01 Apr 2023 00:15 |
Publisher DOI: |
10.1016/j.celrep.2023.112213 |
PubMed ID: |
36870062 |
Uncontrolled Keywords: |
Argonaute CP: Cell biology CP: Molecular biology PTGS Piwi ciliates mRNA clearance siRNAs small RNA clusters small RNAs unicellular untranslated mRNA |
BORIS DOI: |
10.48350/179528 |
URI: |
https://boris.unibe.ch/id/eprint/179528 |
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