Beneke, Tom; Dobramysl, Ulrich; Catta-Preta, Carolina Moura Costa; Mottram, Jeremy Charles; Gluenz, Eva; Wheeler, Richard J (2022). Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase. Wellcome open research, 7(294), p. 294. Wellcome Trust 10.12688/wellcomeopenres.18575.1
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25e88b6b-2efb-4275-815c-d2558d0f0812_18575_-_richard_wheeler__1_.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (423kB) | Preview |
We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology |
UniBE Contributor: |
Glünz, Eva Miriam |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
2398-502X |
Publisher: |
Wellcome Trust |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
07 Mar 2023 14:37 |
Last Modified: |
12 Mar 2023 02:17 |
Publisher DOI: |
10.12688/wellcomeopenres.18575.1 |
PubMed ID: |
36874584 |
Uncontrolled Keywords: |
Genome Leishmania mexicana Pilon polish |
BORIS DOI: |
10.48350/179595 |
URI: |
https://boris.unibe.ch/id/eprint/179595 |
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