Shanbhag, Siddharth; Kampleitner, Carina; Al-Sharabi, Niyaz; Mohamed-Ahmed, Samih; Apaza Alccayhuaman, Karol Ali; Heimel, Patrick; Tangl, Stefan; Beinlich, Andreas; Rana, Neha; Sanz, Mariano; Kristoffersen, Einar K; Mustafa, Kamal; Gruber, Reinhard (2023). Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects. Cells, 12(5) MDPI 10.3390/cells12050767
|
Text
cells-12-00767-v2.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (5MB) | Preview |
Functionalizing biomaterials with conditioned media (CM) from mesenchymal stromal cells (MSC) is a promising strategy for enhancing the outcomes of guided bone regeneration (GBR). This study aimed to evaluate the bone regenerative potential of collagen membranes (MEM) functionalized with CM from human bone marrow MSC (MEM-CM) in critical size rat calvarial defects. MEM-CM prepared via soaking (CM-SOAK) or soaking followed by lyophilization (CM-LYO) were applied to critical size rat calvarial defects. Control treatments included native MEM, MEM with rat MSC (CEL) and no treatment. New bone formation was analyzed via micro-CT (2 and 4 weeks) and histology (4 weeks). Greater radiographic new bone formation occurred at 2 weeks in the CM-LYO group vs. all other groups. After 4 weeks, only the CM-LYO group was superior to the untreated control group, whereas the CM-SOAK, CEL and native MEM groups were similar. Histologically, the regenerated tissues showed a combination of regular new bone and hybrid new bone, which formed within the membrane compartment and was characterized by the incorporation of mineralized MEM fibers. Areas of new bone formation and MEM mineralization were greatest in the CM-LYO group. Proteomic analysis of lyophilized CM revealed the enrichment of several proteins and biological processes related to bone formation. In summary, lyophilized MEM-CM enhanced new bone formation in rat calvarial defects, thus representing a novel 'off-the-shelf' strategy for GBR.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology |
UniBE Contributor: |
Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2073-4409 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
13 Mar 2023 12:18 |
Last Modified: |
13 Mar 2023 23:27 |
Publisher DOI: |
10.3390/cells12050767 |
PubMed ID: |
36899904 |
Uncontrolled Keywords: |
bone tissue engineering conditioned media guided bone regeneration mesenchymal stromal cells regenerative medicine |
BORIS DOI: |
10.48350/179924 |
URI: |
https://boris.unibe.ch/id/eprint/179924 |
Actions (login required)
 |
Edit item |