Hamacher, Jürg; Hadizamani, Yalda; Huwer, Hanno; Moehrlen, Ueli; Bally, Lia; Stammberger, Uz; Wendel, Albrecht; Lucas, Rudolf (2023). Characteristics of inflammatory response and repair after experimental blast lung injury in rats. PLoS ONE, 18(3), e0281446. Public Library of Science 10.1371/journal.pone.0281446
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BACKGROUND AND OBJECTIVES
Blast-induced lung injury is associated with inflammatory, which are characterised by disruption of the alveolar-capillary barrier, haemorrhage, pulmonary infiltrateration causing oedema formation, pro-inflammatory cytokine and chemokine release, and anti-inflammatory counter-regulation. The objective of the current study was to define sequence of such alterations in with establishing blast-induced lung injury in rats using an advanced blast generator.
METHODS
Rats underwent a standardized blast wave trauma and were euthanised at defined time points. Non-traumatised animals served as sham controls. Obtained samples from bronchoalveolar lavage fluid (BALF) at each time-point were assessed for histology, leukocyte infiltration and cytokine/chemokine profile.
RESULTS
After blast lung injury, significant haemorrhage and neutrophil infiltration were observed. Similarly, protein accumulation, lactate dehydrogenase activity (LDH), alveolar eicosanoid release, matrix metalloproteinase (MMP)-2 and -9, pro-Inflammatory cytokines, including tumour necrosis factor (TNF) and interleukin (IL) -6 raised up. While declining in the level of anti-inflammatory cytokine IL-10 occurred. Ultimately, pulmonary oedema developed that increased to its maximum level within the first 1.5 h, then recovered within 24 h.
CONCLUSION
Using a stablished model, can facilitate the study of inflammatory response to blast lung injury. Following the blast injury, alteration in cytokine/chemokine profile and activity of cells in the alveolar space occurs, which eventuates in alveolar epithelial barrier dysfunction and oedema formation. Most of these parameters exhibit time-dependent return to their basal status that is an indication to resilience of lungs to blast-induced lung injury.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Endocrinology, Diabetology and Clinical Nutrition |
UniBE Contributor: |
Hamacher, Jürg, Bally, Lia Claudia |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1932-6203 |
Publisher: |
Public Library of Science |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
20 Mar 2023 14:51 |
Last Modified: |
26 Mar 2023 03:15 |
Publisher DOI: |
10.1371/journal.pone.0281446 |
PubMed ID: |
36928833 |
BORIS DOI: |
10.48350/180300 |
URI: |
https://boris.unibe.ch/id/eprint/180300 |
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