De Lorenzo, Francesca; Lüningschrör, Patrick; Nam, Jinhan; Beckett, Liam; Pilotto, Federica; Galli, Emilia; Lindholm, Päivi; Rüdt von Collenberg, Cora; Tii Mungwa, Simon; Jablonka, Sibylle; Kauder, Julia; Thau-Habermann, Nadine; Petri, Susanne; Lindholm, Dan; Saxena, Smita; Sendtner, Michael; Saarma, Mart; Voutilainen, Merja H (2023). CDNF rescues motor neurons in models of amyotrophic lateral sclerosis by targeting endoplasmic reticulum stress. Brain : a journal of neurology, 146(9), pp. 3783-3799. Oxford University Press 10.1093/brain/awad087
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Amyotrophic lateral sclerosis is a progressive neurodegenerative disease that affects motor neurons (MNs) in the spinal cord, brainstem, and motor cortex, leading to paralysis and eventually to death within 3 to 5 years of symptom onset. To date, no cure or effective therapy is available. The role of chronic endoplasmic reticulum (ER) stress in the pathophysiology of amyotrophic lateral sclerosis, as well as a potential drug target, has received increasing attention. Here, we investigated the mode of action and therapeutic effect of the ER-resident protein cerebral dopamine neurotrophic factor (CDNF) in three preclinical models of amyotrophic lateral sclerosis, exhibiting different disease development and etiology: (i) the conditional choline acetyltransferase (ChAT)-tTA/TRE-hTDP43-M337V rat model previously described, (ii) the widely used SOD1-G93A mouse model, and (iii) a novel slow-progressive TDP43-M337V mouse model. To specifically analyse the ER stress response in MNs, we used three main methods: (i) primary culture of MNs derived from E13 days embryos, (ii) immunohistochemical analyses of spinal cord sections with ChAT as spinal MNs marker, and (iii) qPCR analyses of lumbar MNs isolated via laser microdissection. We show that intracerebroventricular administration of CDNF significantly halts the progression of the disease and improves motor behavior in TDP43-M337V and SOD1-G93A rodent models of amyotrophic lateral sclerosis. CDNF rescues motor neurons in vitro and in vivo from ER stress-associated cell death and its beneficial effect is independent of genetic disease etiology. Notably, CDNF regulates the unfolded protein response (UPR) initiated by transducers IRE1α, PERK, and ATF6, thereby enhancing MN survival. Thus, CDNF holds great promise for the design of new rational treatments for amyotrophic lateral sclerosis.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology |
UniBE Contributor: |
Pilotto, Federica, Saxena, Smita |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1460-2156 |
Publisher: |
Oxford University Press |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
20 Mar 2023 10:45 |
Last Modified: |
02 Sep 2023 00:13 |
Publisher DOI: |
10.1093/brain/awad087 |
PubMed ID: |
36928391 |
Uncontrolled Keywords: |
CDNF ER stress amyotrophic lateral sclerosis motor neurons unfolded protein response |
BORIS DOI: |
10.48350/180302 |
URI: |
https://boris.unibe.ch/id/eprint/180302 |
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