Li, Kaizhen; Figarella, Katherine; Su, Xin; Kovalchuk, Yury; Gorzolka, Jessika; Neher, Jonas J; Mojtahedi, Nima; Casadei, Nicolas; Hedrich, Ulrike B S; Garaschuk, Olga (2023). Endogenous but not sensory-driven activity controls migration, morphogenesis and survival of adult-born juxtaglomerular neurons in the mouse olfactory bulb. Cellular and molecular life sciences : CMLS, 80(4), p. 98. Springer 10.1007/s00018-023-04753-4
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The development and survival of adult-born neurons are believed to be driven by sensory signaling. Here, in vivo analyses of motility, morphology and Ca2+ signaling, as well as transcriptome analyses of adult-born juxtaglomerular cells with reduced endogenous excitability (via cell-specific overexpression of either Kv1.2 or Kir2.1 K+ channels), revealed a pronounced impairment of migration, morphogenesis, survival, and functional integration of these cells into the mouse olfactory bulb, accompanied by a reduction in cytosolic Ca2+ fluctuations, phosphorylation of CREB and pCREB-mediated gene expression. Moreover, K+ channel overexpression strongly downregulated genes involved in neuronal migration, differentiation, and morphogenesis and upregulated apoptosis-related genes, thus locking adult-born cells in an immature and vulnerable state. Surprisingly, cells deprived of sensory-driven activity developed normally. Together, the data reveal signaling pathways connecting the endogenous intermittent neuronal activity/Ca2+ fluctuations as well as enhanced Kv1.2/Kir2.1 K+ channel function to migration, maturation, and survival of adult-born neurons.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Physiology |
UniBE Contributor: |
Li, Kaizhen (A) |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1420-9071 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
20 Mar 2023 11:28 |
Last Modified: |
29 Mar 2023 23:38 |
Publisher DOI: |
10.1007/s00018-023-04753-4 |
PubMed ID: |
36932186 |
Uncontrolled Keywords: |
Adult neurogenesis Differentiation Endogenous activity Migration Neuronal development Olfactory bulb Potassium channels Spontaneous calcium transients Survival pCREB |
BORIS DOI: |
10.48350/180318 |
URI: |
https://boris.unibe.ch/id/eprint/180318 |
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