Pycha, Stefan; D'Elia, Carolina; Mian, Christine; Schwienbacher, Christine; Hanspeter, Esther; Palermo, Margherita; Pycha, Armin; Danuser, Hansjörg; Trenti, Emanuela (2023). Evaluation of the M371-Test Under Real-life Conditions for Diagnosis and Follow Up of Testicular Germ Cell Tumors. Anticancer research, 43(4), pp. 1649-1653. International Inst. of Anticancer Research 10.21873/anticanres.16316
Full text not available from this repository. (Request a copy)BACKGROUND/AIM
The aim of the study was to establish the performance of the M371-Test on the Thermocycler Rotor-GeneQ (Qiagen) platform for diagnosis and follow-up of testicular tumors and to evaluate the test under real-life conditions in comparison to the classical markers alpha-fetoprotein (AFP), beta-human chorionic gonadotropin (β-HCG) and lactate dehydrogenase (LDH).
PATIENTS AND METHODS
Forty-four patients, of median age 29 years (range=24-84) were included in this prospective study at our institution between March 2021 and September 2022. Of the 44 patients, 23 had a suspicion of testicular cancer (TC) and 21 were under follow-up for TC. In total, 96 M371-Tests were performed and compared with AFP, β-HCG, LDH using histological diagnosis and/or computer tomography (CT) scan as the gold standard.
RESULTS
In the patients with suspicion of TC, the M371-Test showed a sensitivity of 73.7%, AFP of 21%, LDH of 31.6% and β-HCG of 42.1%. In the patients under follow-up for TC, the M371-Test showed a sensitivity of 86.4%, AFP of 50%, LDH of 31.8% and β-HCG of 63.6%. In germ cell tumours (GCT)/non-seminomas, M371-Test had a sensitivity of 83.3%, AFP of 77.8%, LDH of 38.9% and β-HCG of 66.7%. In GCT/seminomas, M371-Test had a sensitivity of 85%, AFP of 5%, LDH of 30% and β-HCG of 50%.
CONCLUSION
Under real life conditions performed on the real-time Thermocycler Rotor-GeneQ (Qiagen) platform, the M371-Test shows an outstanding performance and is far beyond the sensitivity of the classical markers for detecting GCTs and in the follow-up of patients after GCT, especially in seminomas.
Item Type: |
Journal Article (Original Article) |
|---|---|
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0250-7005 |
Publisher: |
International Inst. of Anticancer Research |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
29 Mar 2023 08:19 |
Last Modified: |
29 Mar 2023 08:19 |
Publisher DOI: |
10.21873/anticanres.16316 |
PubMed ID: |
36974797 |
Additional Information: |
Stefan Pycha hat keine Rolle, Affiliation unvollständig |
Uncontrolled Keywords: |
Germ cell tumors M371-test miRNA testicular |
URI: |
https://boris.unibe.ch/id/eprint/180905 |
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