CuMV VLPs Containing the RBM from SARS-CoV-2 Spike Protein Drive Dendritic Cell Activation and Th1 Polarization.

Sebastião, Ana Isabel; Mateus, Daniela; Carrascal, Mylène A; Sousa, Cátia; Cortes, Luísa; Bachmann, Martin F; do Carmo, Anália; Matos, Ana Miguel; Sales, Maria Goreti F; Cruz, Maria Teresa (2023). CuMV VLPs Containing the RBM from SARS-CoV-2 Spike Protein Drive Dendritic Cell Activation and Th1 Polarization. Pharmaceutics, 15(3) MDPI 10.3390/pharmaceutics15030825

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Dendritic cells (DCs) are the most specialized and proficient antigen-presenting cells. They bridge innate and adaptive immunity and display a powerful capacity to prime antigen-specific T cells. The interaction of DCs with the receptor-binding domain of the spike (S) protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a pivotal step to induce effective immunity against the S protein-based vaccination protocols, as well as the SARS-CoV-2 virus. Herein, we describe the cellular and molecular events triggered by virus-like particles (VLPs) containing the receptor-binding motif from the SARS-CoV-2 spike protein in human monocyte-derived dendritic cells, or, as controls, in the presence of the Toll-like receptors (TLR)3 and TLR7/8 agonists, comprehending the events of dendritic cell maturation and their crosstalk with T cells. The results demonstrated that VLPs boosted the expression of major histocompatibility complex molecules and co-stimulatory receptors of DCs, indicating their maturation. Furthermore, DCs' interaction with VLPs promoted the activation of the NF-kB pathway, a very important intracellular signalling pathway responsible for triggering the expression and secretion of proinflammatory cytokines. Additionally, co-culture of DCs with T cells triggered CD4+ (mainly CD4+Tbet+) and CD8+ T cell proliferation. Our results suggested that VLPs increase cellular immunity, involving DC maturation and T cell polarization towards a type 1 T cells profile. By providing deeper insight into the mechanisms of activation and regulation of the immune system by DCs, these findings will enable the design of effective vaccines against SARS-CoV-2.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Rheumatologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Rheumatologie

UniBE Contributor:

Bachmann, Martin (B)

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1999-4923

Publisher:

MDPI

Language:

English

Submitter:

Pubmed Import

Date Deposited:

30 Mar 2023 14:45

Last Modified:

25 Feb 2024 06:02

Publisher DOI:

10.3390/pharmaceutics15030825

PubMed ID:

36986686

Uncontrolled Keywords:

COVID-19 SARS-CoV-2 adaptive immune response dendritic cells memory T cell virus-like particles

BORIS DOI:

10.48350/181067

URI:

https://boris.unibe.ch/id/eprint/181067

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