Caldwell, Jessica; Loussert-Fonta, Céline; Toullec, Gaëlle; Heidelberg Lyndby, Niclas; Haenni, Beat; Taladriz-Blanco, Patricia; Espiña, Begoña; Rothen-Rutishauser, Barbara; Petri-Fink, Alke (2023). Correlative Light, Electron Microscopy and Raman Spectroscopy Workflow To Detect and Observe Microplastic Interactions with Whole Jellyfish. Environmental science & technology, 57(16), pp. 6664-6672. ACS Publications 10.1021/acs.est.2c09233
|
Text
acs.est.2c09233.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (7MB) | Preview |
Many researchers have turned their attention to understanding microplastic interaction with marine fauna. Efforts are being made to monitor exposure pathways and concentrations and to assess the impact such interactions may have. To answer these questions, it is important to select appropriate experimental parameters and analytical protocols. This study focuses on medusae of Cassiopea andromeda jellyfish: a unique benthic jellyfish known to favor (sub-)tropical coastal regions which are potentially exposed to plastic waste from land-based sources. Juvenile medusae were exposed to fluorescent poly(ethylene terephthalate) and polypropylene microplastics (<300 μm), resin embedded, and sectioned before analysis with confocal laser scanning microscopy as well as transmission electron microscopy and Raman spectroscopy. Results show that the fluorescent microplastics were stable enough to be detected with the optimized analytical protocol presented and that their observed interaction with medusae occurs in a manner which is likely driven by the microplastic properties (e.g., density and hydrophobicity).
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
09 Interdisciplinary Units > Microscopy Imaging Center (MIC) 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy |
UniBE Contributor: |
Haenni, Beat |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0013-936X |
Publisher: |
ACS Publications |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
17 Apr 2023 12:10 |
Last Modified: |
26 Apr 2023 00:16 |
Publisher DOI: |
10.1021/acs.est.2c09233 |
PubMed ID: |
37058431 |
Uncontrolled Keywords: |
Cassiopea andromeda Raman spectroscopy correlative microscopy medusa microplastics |
BORIS DOI: |
10.48350/181732 |
URI: |
https://boris.unibe.ch/id/eprint/181732 |
Actions (login required)
 |
Edit item |