Vanheuverzwijn, Jérome; Maillard, Eloise-Eliane; Mahat, Amal; Fowler, Lee; Monteyne, Daniel; Bonnaud, Leïla; Landercy, Nicolas; Hemberg, Axel; Janković, Ana; Meyer, Franck; Mišković-Stanković, Vesna; Stevanović, Milena; Mirica, Codruta; Pérez-Morga, David; Luginbühl, Reto; Combes, Christèle; Furtos, Gabriel; Fontaine, Véronique (2023). Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials. Microorganisms, 11(4) MDPI 10.3390/microorganisms11041023
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Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either S. aureus or P. aeruginosa as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) |
UniBE Contributor: |
Luginbühl, Reto |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2076-2607 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
01 May 2023 13:01 |
Last Modified: |
02 May 2023 03:26 |
Publisher DOI: |
10.3390/microorganisms11041023 |
PubMed ID: |
37110446 |
Uncontrolled Keywords: |
anti-biofilm copper infection medical device metal ion surface |
BORIS DOI: |
10.48350/182097 |
URI: |
https://boris.unibe.ch/id/eprint/182097 |
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