Sgammeglia, Noemi; Widmer, Yves F; Kaldun, Jenifer C; Fritsch, Cornelia; Bruggmann, Rémy; Sprecher, Simon G (2023). Memory phase-specific genes in the Mushroom Bodies identified using CrebB-target DamID. PLoS genetics, 19(6), e1010802. Public Library of Science 10.1371/journal.pgen.1010802
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The formation of long-term memories requires changes in the transcriptional program and de novo protein synthesis. One of the critical regulators for long-term memory (LTM) formation and maintenance is the transcription factor CREB. Genetic studies have dissected the requirement of CREB activity within memory circuits, however less is known about the genetic mechanisms acting downstream of CREB and how they may contribute defining LTM phases. To better understand the downstream mechanisms, we here used a targeted DamID approach (TaDa). We generated a CREB-Dam fusion protein using the fruit fly Drosophila melanogaster as model. Expressing CREB-Dam in the mushroom bodies (MBs), a brain center implicated in olfactory memory formation, we identified genes that are differentially expressed between paired and unpaired appetitive training paradigm. Of those genes we selected candidates for an RNAi screen in which we identified genes causing increased or decreased LTM.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Bioinformatics and Computational Biology |
UniBE Contributor: |
Bruggmann, Rémy |
ISSN: |
1553-7404 |
Publisher: |
Public Library of Science |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
13 Jun 2023 09:25 |
Last Modified: |
25 Jun 2023 00:20 |
Publisher DOI: |
10.1371/journal.pgen.1010802 |
PubMed ID: |
37307281 |
BORIS DOI: |
10.48350/183359 |
URI: |
https://boris.unibe.ch/id/eprint/183359 |
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