Arnold, Kim; Dehio, Philippe; Lötscher, Jonas; Singh, Kapil Dev; García-Gómez, Diego; Hess, Christoph; Sinues, Pablo; Balmer, Maria L (2023). Real-Time Volatile Metabolomics Analysis of Dendritic Cells. Analytical chemistry, 95(25), pp. 9415-9421. American Chemical Society 10.1021/acs.analchem.3c00516
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Dendritic cells (DCs) actively sample and present antigen to cells of the adaptive immune system and are thus vital for successful immune control and memory formation. Immune cell metabolism and function are tightly interlinked, and a better understanding of this interaction offers potential to develop immunomodulatory strategies. However, current approaches for assessing the immune cell metabolome are often limited by end-point measurements, may involve laborious sample preparation, and may lack unbiased, temporal resolution of the metabolome. In this study, we present a novel setup coupled to a secondary electrospray ionization-high resolution mass spectrometric (SESI-HRMS) platform allowing headspace analysis of immature and activated DCs in real-time with minimal sample preparation and intervention, with high technical reproducibility and potential for automation. Distinct metabolic signatures of DCs treated with different supernatants (SNs) of bacterial cultures were detected during real-time analyses over 6 h compared to their respective controls (SN only). Furthermore, the technique allowed for the detection of 13C-incorporation into volatile metabolites, opening the possibility for real-time tracing of metabolic pathways in DCs. Moreover, differences in the metabolic profile of naı̈ve and activated DCs were discovered, and pathway-enrichment analysis revealed three significantly altered pathways, including the TCA cycle, α-linolenic acid metabolism, and valine, leucine, and isoleucine degradation.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Endokrinologie / Diabetologie (Erwachsene) 04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Endocrinology, Diabetology and Clinical Nutrition |
UniBE Contributor: |
Balmer, Maria Luisa |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1520-6882 |
Publisher: |
American Chemical Society |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
14 Jun 2023 09:19 |
Last Modified: |
28 Jun 2023 00:16 |
Publisher DOI: |
10.1021/acs.analchem.3c00516 |
PubMed ID: |
37311562 |
BORIS DOI: |
10.48350/183406 |
URI: |
https://boris.unibe.ch/id/eprint/183406 |
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