Cansever, Dilay; Petrova, Ekaterina; Krishnarajah, Sinduya; Mussak, Caroline; Welsh, Christina A; Mildenberger, Wiebke; Mulder, Kevin; Kreiner, Victor; Roussel, Elsa; Stifter, Sebastian A; Andreadou, Myrto; Zwicky, Pascale; Jurado, Nicole Puertas; Rehrauer, Hubert; Tan, Ge; Liu, Zhaoyuan; Blériot, Camille; Ronchi, Francesca; Macpherson, Andrew J; Ginhoux, Florent; ... (2023). Lactation-associated macrophages exist in murine mammary tissue and human milk. Nature immunology, 24(7), pp. 1098-1109. Springer Nature 10.1038/s41590-023-01530-0
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Macrophages are involved in immune defense, organogenesis and tissue homeostasis. Macrophages contribute to the different phases of mammary gland remodeling during development, pregnancy and involution postlactation. Less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multiparameter flow cytometry and single-cell RNA sequencing, we identified a lactation-induced CD11c+CX3CR1+Dectin-1+ macrophage population (liMac) that was distinct from the two resident F4/80hi and F4/80lo macrophage subsets present pregestationally. LiMacs were predominantly monocyte-derived and expanded by proliferation in situ concomitant with nursing. LiMacs developed independently of IL-34, but required CSF-1 signaling and were partly microbiota-dependent. Locally, they resided adjacent to the basal cells of the alveoli and extravasated into the milk. We found several macrophage subsets in human milk that resembled liMacs. Collectively, these findings reveal the emergence of unique macrophages in the mammary gland and milk during lactation.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Gastroenterology 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine |
UniBE Contributor: |
Ronchi, Francesca, Macpherson, Andrew |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1529-2916 |
Publisher: |
Springer Nature |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
20 Jun 2023 12:20 |
Last Modified: |
30 Jun 2023 00:16 |
Publisher DOI: |
10.1038/s41590-023-01530-0 |
PubMed ID: |
37337103 |
BORIS DOI: |
10.48350/183545 |
URI: |
https://boris.unibe.ch/id/eprint/183545 |
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