Lu, Xia; Zhu, Meilin; Zhao, Lingzhou; Qi, Feiran; Zou, Heng; He, Peng; Zhou, Haizhong; Shi, Kuangyu; Du, Jie (2023). 68Ga-labeled WVP peptide as a novel PET probe for molecular biological diagnosis of unstable thoracic aortic aneurysm and early dissection: an animal study. Frontiers in cardiovascular medicine, 10(1048927), p. 1048927. Frontiers 10.3389/fcvm.2023.1048927
|
Text
fcvm-10-1048927.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (7MB) | Preview |
OBJECTIVE
Type IV collagen (Col-IV) is a prospective biomarker for diagnosing and treating of unstable thoracic aortic aneurysm and dissection (TAAD). This study aims to evaluate the feasibility of 68Ga-labeled WVP peptide (68Ga-DOTA-WVP) as a novel Col-IV-targeted probe for TAAD biological diagnosis using PET/CT.
METHODS
WVP peptide was modified with bifunctional chelator DOTA for 68Ga radiolabeling. Immunohistochemical staining was used to evaluate the expression and location of Col-IV and elastin in aortas treated with 3-aminopropionitrile fumarate (BAPN) at different time points (0, 2, and 4 weeks). The imaging performance of 68Ga-DOTA-WVP was investigated using Micro-PET/CT in a BAPN-induced TAAD mouse model. The relationship between 68Ga-DOTA-WVP uptake in aortic lesions and the serum levels of TAAD-related biomarkers including D-dimer, C-reactive protein (CRP), and serum soluble suppression of tumorigenicity-2 (sST2) was also analyzed.
RESULTS
68Ga-DOTA-WVP was readily prepared with high radiochemical purity and stability in vitro. 68Ga-DOTA-WVP Micro-PET/CT could detect Col-IV exposure of unstable aneurysms and early dissection in BAPN-induced TAAD mice, but little 68Ga-DOTA-WVP uptake was shown in the control group at each imaging time point. The differences of Col-IV expression and distribution of 68Ga-DOTA-WVP both in TAAD and control groups further verified the imaging efficiency of 68Ga-DOTA-WVP PET/CT. Additionally, a higher sST2 level was found in the imaging positive (n = 14) than the negative (n = 8) group (9.60 ± 1.14 vs. 8.44 ± 0.52, P = 0.014).
CONCLUSION
68Ga-DOTA-WVP could trace the exposure and abnormal deposition of Col-IV in enlarged and early injured aortas, showing a potential for biological diagnosis, whole-body screening, and progression monitoring of TAAD.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Clinic of Nuclear Medicine |
UniBE Contributor: |
Shi, Kuangyu |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2297-055X |
Publisher: |
Frontiers |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
30 Jun 2023 11:05 |
Last Modified: |
16 Jul 2023 02:26 |
Publisher DOI: |
10.3389/fcvm.2023.1048927 |
PubMed ID: |
37378402 |
Uncontrolled Keywords: |
PET/CT imaging WVP peptide molecular biological diagnosis thoracic aortic aneurysm and dissection type IV collagen detection |
BORIS DOI: |
10.48350/184225 |
URI: |
https://boris.unibe.ch/id/eprint/184225 |
Actions (login required)
 |
Edit item |