Viral load, organ distribution, histopathological lesions, and cytokine mRNA expression in goats infected with a molecular clone of the caprine arthritis encephalitis virus

Ravazzolo, Ana Paula; Nenci, Chiara; Vogt, Hans-Rudolf; Waldvogel, Andreas; Obexer-Ruff, Gaby; Peterhans, Ernst; Bertoni, Giuseppe (2006). Viral load, organ distribution, histopathological lesions, and cytokine mRNA expression in goats infected with a molecular clone of the caprine arthritis encephalitis virus. Virology, 350(1), pp. 116-27. New York, N.Y.: Academic Press 10.1016/j.virol.2006.02.014

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Caprine arthritis encephalitis virus (CAEV) is a lentivirus of goats that causes persistent infection characterized by the appearance of inflammatory lesions in various organs. To define the sites of persistence, 5 goats were infected with a molecular clone of CAEV, and the viral load was monitored by real-time-PCR and RT-PCR in different sites 8 years after infection. The lymph nodes proved to be an important virus reservoir, with moderate virus replication relative to what is reported for lentiviruses of primates. Mammary gland and milk cells were preferred sites of viral replication. The viral load varied significantly between animals, which points to an important role of the genetic background. We found a clear association between occurrence of histopathological lesions and viral load in specific sites. The mRNA expression analysis of several cytokines did not reveal differences between animals that could explain the considerable individual variations in viral load observed.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics

UniBE Contributor:

Nenci, Chiara; Vogt, Hans-Rudolf; Obexer, Gaby; Peterhans, Ernst and Bertoni, Giuseppe

ISSN:

0042-6822

Publisher:

Academic Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

04 May 2014 23:13

Publisher DOI:

10.1016/j.virol.2006.02.014

PubMed ID:

16537085

Web of Science ID:

000238561900011

URI:

https://boris.unibe.ch/id/eprint/18433 (FactScience: 514)

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