Analysis of the antibody response to an immunodominant epitope of the envelope glycoprotein of a lentivirus and its diagnostic potential

Mordasini, Franca; Vogt, Hans-Rudolf; Zahno, Marie-Luise; Maeschli, Ariane; Nenci, Chiara; Zanoni, Reto; Peterhans, Ernst; Bertoni, Giuseppe (2006). Analysis of the antibody response to an immunodominant epitope of the envelope glycoprotein of a lentivirus and its diagnostic potential. Journal of clinical microbiology, 44(3), pp. 981-91. Washington, D.C.: American Society for Microbiology 10.1128/JCM.44.3.981-991.2006

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The envelope glycoprotein of small ruminant lentiviruses (SRLV) is a major target of the humoral immune response and contains several linear B-cell epitopes. We amplified and sequenced the genomic segment encoding the SU5 antigenic site of the envelope glycoprotein of several SRLV field isolates. With synthetic peptides based on the deduced amino acid sequences of SU5 in an enzyme-linked immunosorbent assay (ELISA), we have (i) proved the immunodominance of this region regardless of its high variability, (ii) defined the epitopes encompassed by SU5, (iii) illustrated the rapid and peculiar kinetics of seroconversion to this antigenic site, and (iv) shown the rapid and strong maturation of the avidity of the anti-SU5 antibody. Finally, we demonstrated the modular diagnostic potential of SU5 peptides. Under Swiss field conditions, the SU5 ELISA was shown to detect the majority of infected animals and, when applied in a molecular epidemiological context, to permit rapid phylogenetic classification of the infecting virus.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology

UniBE Contributor:

Vogt, Hans-Rudolf; Zahno, Marie-Luise; Nenci, Chiara; Zanoni, Reto Giacomo; Peterhans, Ernst and Bertoni, Giuseppe

ISSN:

0095-1137

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

04 May 2014 23:13

Publisher DOI:

10.1128/JCM.44.3.981-991.2006

PubMed ID:

16517887

Web of Science ID:

000236095000049

URI:

https://boris.unibe.ch/id/eprint/18434 (FactScience: 515)

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