Ferreira Sávio, Débora de Souza; Silva, Lucia Moitrel Pequeno da; Reis, Gabriel Guerra David; Denardi, Ricardo Junior; Costa, Natacha Malu Miranda da; Chaves Furlaneto, Flávia Aparecida; Souza, Sérgio Luís Scombatti de; Mourão, Carlos Fernando de Almeida Barros; Miron, Richard J; Okamoto, Roberta; Messora, Michel Reis (2023). Effects of platelet-rich fibrin produced by three centrifugation protocols on bone neoformation in defects created in rat calvaria. Platelets, 34(1), p. 2228417. Tayler & Francis 10.1080/09537104.2023.2228417
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Effects_of_platelet_rich_fibrin_produced_by_three_centrifugation_protocols_on_bone_neoformation_in_defects_created_in_rat_calvaria.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (9MB) | Preview |
This study evaluated the potential of Leukocyte-platelet-rich fibrin (L-PRF; fixed angle centrifugation protocol), Advanced-platelet-rich fibrin (A-PRF; low-speed fixed angle centrifugation protocol), and Horizontal-platelet-rich fibrin (H-PRF; horizontal centrifugation protocol) in bone neoformation in critical size defects (CSDs) in rat calvaria. Thirty-two rats were divided into groups: Control (C), L-PRF, A-PRF, and H-PRF. 5 mm diameter CSDs were created in the animals' calvaria. Defects from group Control (C) were filled with blood clots, while defects from groups L-PRF, A-PRF, and H-PRF were filled with respective platelet-rich fibrin (PRF) membranes. L-PRF, A-PRF, and H-PRF were prepared from animal blood collection and specific centrifugation protocols. At 14 and 30 days, calcein (CA) and alizarin (AL) injections were performed, respectively. Animals were euthanized at 35 days. Microtomographic, laser confocal microscopy, and histomorphometric analyzes were performed. Data were statistically analyzed (ANOVA, Tukey, p < .05). L-PRF, A-PRF, and H-PRF groups showed higher values of bone volume (BV), newly formed bone area (NFBA), and precipitation of CA and AL than the C group (p < .05). The H-PRF group showed higher values of BV, number of trabeculae (Tb. N), NFBA, and higher precipitation of AL than the A-PRF and L-PRF groups (p < .05). Therefore, it can be concluded that: i) L-PRF, A-PRF, and H-PRF potentiate bone neoformation in CSDs in rat calvaria; ii) H-PRF demonstrated more biological potential for bone healing.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology |
UniBE Contributor: |
Miron, Richard John |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1369-1635 |
Publisher: |
Tayler & Francis |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
06 Jul 2023 15:24 |
Last Modified: |
16 Jul 2023 02:27 |
Publisher DOI: |
10.1080/09537104.2023.2228417 |
PubMed ID: |
37409489 |
Uncontrolled Keywords: |
Bone regeneration, platelet-rich fibrin, rats |
BORIS DOI: |
10.48350/184548 |
URI: |
https://boris.unibe.ch/id/eprint/184548 |
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