Bonnet, Diane Marie Valerie; Tirot, Louis; Grob, Stefan; Jullien, Pauline Emilie (2023). Methylome response to proteasome inhibition by the Pseudomonas syringae virulence factor Syringolin A. Molecular Plant-Microbe Interactions, 36(11), pp. 693-704. American Phytopathological Society 10.1094/MPMI-06-23-0080-R
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DNA methylation is an important epigenetic mark required for proper gene expression and silencing of transposable elements. DNA methylation patterns can be modified by environmental factors such as pathogen infection, where modification of DNA methylation can be associated with plant resistance. To counter the plant defense pathways, pathogens produce effector molecules, several of which act as proteasome inhibitors. Here we investigated the effect of proteasome inhibition by the bacterial virulence factor Syringolin A on genome-wide DNA methylation. We show that Syringolin A treatment results in an increase of DNA methylation at centromeric and pericentromeric regions of Arabidopsis chromosomes. We identify several CHH DMRs that are enriched in the proximity of transcriptional start sites. Syringolin A treatment does not result in significant changes in small RNA composition. However, significant changes in genome transcriptional activity can be observed, including a strong upregulation of resistance genes that are located on chromosomal arms. We hypothesize that DNA methylation changes could be linked to the upregulation of some atypical members of the de novo DNA methylation pathway: AGO3, AGO9 and DRM1. Our data suggests that modification of genome-wide DNA methylation resulting from an inhibition of the proteasome by bacterial effectors could be part of an epi-genomic arms race against pathogens.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Epigenetics 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) |
UniBE Contributor: |
Bonnet, Diane Marie Valérie J., Tirot, Louis Jean François, Jullien, Pauline Emilie |
Subjects: |
500 Science > 580 Plants (Botany) |
ISSN: |
0894-0282 |
Publisher: |
American Phytopathological Society |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
10 Jul 2023 14:02 |
Last Modified: |
18 Dec 2023 15:54 |
Publisher DOI: |
10.1094/MPMI-06-23-0080-R |
PubMed ID: |
37414416 |
BORIS DOI: |
10.48350/184566 |
URI: |
https://boris.unibe.ch/id/eprint/184566 |
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