Sequence analysis of a 212 kb defensin gene cluster on ECA 27q17

Looft, Christian; Paul, Sven; Philipp, Ute; Regenhard, Petra; Kuiper, Heidi; Distl, Ottmar; Chowdhary, Bhanu P; Leeb, Tosso (2006). Sequence analysis of a 212 kb defensin gene cluster on ECA 27q17. Gene, 376(2), pp. 192-8. Amsterdam: Elsevier 10.1016/j.gene.2006.03.006

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Defensins are a family of evolutionary ancient antimicrobial peptides consisting of three sub-families: alpha-, beta- and theta-defensins. This investigation was focused on the genomic characterization of equine beta-defensins and the investigation of the potential clustering of beta-defensin genes in the equine genome. Six genomic BAC clones were isolated from the CHORI-241 library and one of these was mapped by FISH to ECA 27q17. This location was confirmed by RH-mapping. The contiguous 212 kb sequence of this clone was determined. Sequence analysis revealed the identification of ten pseudogenes and nine genes, six of which were highly homologous to human beta-defensin DEFB4. Clustering of the beta-defensin genes was confirmed and the order of the genes on the analyzed BAC was related to the corresponding defensin cluster on HSA 8. The knowledge about the sequence and the genomic structure of the equine beta-defensin genes will improve the classification of different paralogous defensin genes and is a prerequisite for subsequent functional studies. Additionally, the first alpha-defensin-like sequence outside the groups of primates, lagomorphs and rodents (glires) was identified.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics

UniBE Contributor:

Leeb, Tosso

ISSN:

0378-1119

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

04 May 2014 23:13

Publisher DOI:

10.1016/j.gene.2006.03.006

PubMed ID:

16723195

Web of Science ID:

000239357700004

URI:

https://boris.unibe.ch/id/eprint/18462 (FactScience: 605)

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