Momotake, Atsuya; Lindegger, Nicolas; Niggli, Ernst; Barsotti, Robert J; Ellis-Davies, Graham C R (2006). The nitrodibenzofuran chromophore: a new caging group for ultra-efficient photolysis in living cells. Nature methods, 3(1), pp. 35-40. New York, N.Y.: Nature Publishing Group 10.1038/NMETH821
Full text not available from this repository. (Request a copy)Photochemical uncaging of bio-active molecules was introduced in 1977, but since then, there has been no substantial improvement in the properties of generic caging chromophores. We have developed a new chromophore, nitrodibenzofuran (NDBF) for ultra-efficient uncaging of second messengers inside cells. Photolysis of a NDBF derivative of EGTA (caged calcium) is about 16-160 times more efficient than photolysis of the most widely used caged compounds (the quantum yield of photolysis is 0.7 and the extinction coefficient is 18,400 M(-1) cm(-1)). Ultraviolet (UV)-laser photolysis of NDBF-EGTA:Ca(2+) rapidly released Ca(2+) (rate of 20,000 s(-1)) and initiated contraction of skinned guinea pig cardiac muscle. NDBF-EGTA has a two-photon cross-section of approximately 0.6 GM and two-photon photolysis induced localized Ca(2+)-induced Ca(2+) release from the sarcoplasmic recticulum of intact cardiac myocytes. Thus, the NDBF chromophore has great promise as a generic and photochemically efficient protecting group for both one- and two-photon uncaging in living cells.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Physiology |
UniBE Contributor: |
Lindegger, Nicolas, Niggli, Ernst |
ISSN: |
1548-7091 |
ISBN: |
16369551 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:45 |
Last Modified: |
05 Dec 2022 14:14 |
Publisher DOI: |
10.1038/NMETH821 |
PubMed ID: |
16369551 |
Web of Science ID: |
000234528000015 |
URI: |
https://boris.unibe.ch/id/eprint/18522 (FactScience: 702) |
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