Improved protocol for chromatofocusing on the ProteomeLab PF2D

Barré, Olivier; Solioz, Marc (2006). Improved protocol for chromatofocusing on the ProteomeLab PF2D. Proteomics, 6(19), pp. 5096-8. Weinheim: Wiley-VCH 10.1002/pmic.200600074

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Beckman-Coulter has recently introduced the ProteomeLab PF2D for 2-D liquid separation of protein samples. The system features separation in the first dimension by chromatofocusing, followed by RP chromatography in the second dimension, allowing the analysis of complex proteomics samples. When used by the standard protocol, reproducibility and column life times are limited, making the use of the instrument very costly. We here present an improved protocol for chromatofocusing, which enhances column life by at least fivefold.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Clinical Pharmacology and Visceral Research [discontinued]

UniBE Contributor:

Barré, Olivier and Solioz, Marc

ISSN:

1615-9853

ISBN:

16941567

Publisher:

Wiley-VCH

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

06 Dec 2013 13:41

Publisher DOI:

10.1002/pmic.200600074

PubMed ID:

16941567

Web of Science ID:

000241249800002

URI:

https://boris.unibe.ch/id/eprint/18562 (FactScience: 754)

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