Ganguin, Aymar Abel; Skorup, Ivo; Streb, Sebastian; Othman, Alaa; Luciani, Paola (2023). Formation and Investigation of Cell-Derived Nanovesicles as Potential Therapeutics Against Chronic Liver Disease. Advanced healthcare materials, 12(30), e2300811. Wiley 10.1002/adhm.202300811
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This study aims to offer a new therapeutic approach using cell-derived nanovesicles (cdNVs) to overcome the lack of effective treatments for liver fibrosis, a reversible chronic liver disease. To achieve this goal we established the formation and purification of cdNVs from untreated, quiescent-like, or activated LX-2 cells, an immortalized human hepatic stellate cell (HSC) line with key features of transdifferentiated HSCs. Analysis of the genotype and phenotype of naïve and transdifferentiated LX-2 cells activated through transforming growth factor beta 1 (TGF-β1 ), following treatment with cdNVs, revealed a concentration-dependent fibrosis regression. The beneficial fibrosis-resolving effects of cdNVs are linked to their biomolecular corona. Liposomes generated using lipids extracted from cdNVs exhibit a reduced anti-fibrotic response in perpetuated LX-2 cells and show a reduced cellular uptake. However, incubation with soluble factors collected during purification results in a new corona, thereby restoring fibrosis regression activity. Overall, cdNVs display encouraging therapeutic properties, making them a promising candidate for the development of liver fibrosis resolving therapeutics. This article is protected by copyright. All rights reserved.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Ganguin, Aymar Abel, Skorup, Ivo, Luciani, Paola |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry 000 Computer science, knowledge & systems |
ISSN: |
2192-2659 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
06 Sep 2023 10:27 |
Last Modified: |
05 Dec 2023 00:13 |
Publisher DOI: |
10.1002/adhm.202300811 |
PubMed ID: |
37669775 |
Uncontrolled Keywords: |
LX-2 cells anti-fibrotic potential cell-derived nanovesicles (cdNVs) corona |
BORIS DOI: |
10.48350/186082 |
URI: |
https://boris.unibe.ch/id/eprint/186082 |
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