de Araujo, Leonardo; Cabibbe, Andrea Maurizio; Mhuulu, Lusia; Ruswa, Nunurai; Dreyer, Viola; Diergaardt, Azaria; Günther, Gunar; Claassens, Mareli; Gerlach, Christiane; Utpatel, Christian; Cirillo, Daniela Maria; Nepolo, Emmanuel; Niemann, Stefan (2023). Implementation of targeted next-generation sequencing for the diagnosis of drug-resistant tuberculosis in low-resource settings: a programmatic model, challenges, and initial outcomes. Frontiers in Public Health, 11, p. 1204064. Frontiers Research Foundation 10.3389/fpubh.2023.1204064
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Targeted next-generation sequencing (tNGS) from clinical specimens has the potential to become a comprehensive tool for routine drug-resistance (DR) prediction of Mycobacterium tuberculosis complex strains (MTBC), the causative agent of tuberculosis (TB). However, TB mainly affects low- and middle-income countries, in which the implementation of new technologies have specific needs and challenges. We propose a model for programmatic implementation of tNGS in settings with no or low previous sequencing capacity/experience. We highlight the major challenges and considerations for a successful implementation. This model has been applied to build NGS capacity in Namibia, an upper middle-income country located in Southern Africa and suffering from a high-burden of TB and TB-HIV, and we describe herein the outcomes of this process.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology |
UniBE Contributor: |
Günther, Gunar |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2296-2565 |
Publisher: |
Frontiers Research Foundation |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
07 Sep 2023 16:13 |
Last Modified: |
29 Oct 2023 02:22 |
Publisher DOI: |
10.3389/fpubh.2023.1204064 |
PubMed ID: |
37674674 |
Uncontrolled Keywords: |
Genomic diagnostic Genomic surveillance Mycobacterium tuberculosis complex (MTBC) NGS clinical use NGS programmatic implementation Next generation sequencing (NGS) high TB burden countries low- and middle-income countries |
BORIS DOI: |
10.48350/186136 |
URI: |
https://boris.unibe.ch/id/eprint/186136 |
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