Mapunda, Josephine A; Pareja, Javier; Vladymyrov, Mykhailo; Bouillet, Elisa; Hélie, Pauline; Pleskač, Petr; Barcos, Sara; Andrae, Johanna; Vestweber, Dietmar; McDonald, Donald M; Betsholtz, Christer; Deutsch, Urban; Proulx, Steven T; Engelhardt, Britta (2023). VE-cadherin in arachnoid and pia mater cells serves as a suitable landmark for in vivo imaging of CNS immune surveillance and inflammation. Nature communications, 14(1), p. 5837. Nature Publishing Group 10.1038/s41467-023-41580-4
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Meninges cover the surface of the brain and spinal cord and contribute to protection and immune surveillance of the central nervous system (CNS). How the meningeal layers establish CNS compartments with different accessibility to immune cells and immune mediators is, however, not well understood. Here, using 2-photon imaging in female transgenic reporter mice, we describe VE-cadherin at intercellular junctions of arachnoid and pia mater cells that form the leptomeninges and border the subarachnoid space (SAS) filled with cerebrospinal fluid (CSF). VE-cadherin expression also marked a layer of Prox1+ cells located within the arachnoid beneath and separate from E-cadherin+ arachnoid barrier cells. In vivo imaging of the spinal cord and brain in female VE-cadherin-GFP reporter mice allowed for direct observation of accessibility of CSF derived tracers and T cells into the SAS bordered by the arachnoid and pia mater during health and neuroinflammation, and detection of volume changes of the SAS during CNS pathology. Together, the findings identified VE-cadherin as an informative landmark for in vivo imaging of the leptomeninges that can be used to visualize the borders of the SAS and thus potential barrier properties of the leptomeninges in controlling access of immune mediators and immune cells into the CNS during health and neuroinflammation.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute 09 Interdisciplinary Units > Microscopy Imaging Center (MIC) |
UniBE Contributor: |
Mapunda, Josephine Angelo, Pareja Román, Javier, Vladymyrov, Mykhailo, Bouillet, Elisa Catherine, Hélie, Pauline Charlotte Andrée, Pleskac, Petr, Barcos, Sara, Deutsch, Urban, Proulx, Steven Thomas, Engelhardt, Britta |
Subjects: |
600 Technology > 610 Medicine & health 500 Science |
ISSN: |
2041-1723 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
21 Sep 2023 10:03 |
Last Modified: |
29 Oct 2023 02:24 |
Publisher DOI: |
10.1038/s41467-023-41580-4 |
PubMed ID: |
37730744 |
BORIS DOI: |
10.48350/186443 |
URI: |
https://boris.unibe.ch/id/eprint/186443 |
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