Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

Korczak, Bozena M; Stieber, Regina; Emler, Stefan; Burnens, André P; Frey, Joachim; Kuhnert, Peter (2006). Genetic relatedness within the genus Campylobacter inferred from rpoB sequences. International journal of systematic and evolutionary microbiology, 56(Pt 5), pp. 937-45. Reading, UK: Society for General Microbiology SGM 10.1099/ijs.0.64109-0

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The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Korczak, Bozena; Frey, Joachim and Kuhnert, Peter

ISSN:

1466-5026

Publisher:

Society for General Microbiology SGM

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

21 Jan 2014 15:06

Publisher DOI:

10.1099/ijs.0.64109-0

PubMed ID:

16627635

URI:

https://boris.unibe.ch/id/eprint/18654 (FactScience: 859)

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