Distribution of RTX toxin genes in strains of [Actinobacillus] rossii and [Pasteurella] mairii

Mayor, Désirée; Korczak, Bozena M; Christensen, Henrik; Bisgaard, Magne; Frey, Joachim; Kuhnert, Peter (2006). Distribution of RTX toxin genes in strains of [Actinobacillus] rossii and [Pasteurella] mairii. Veterinary microbiology, 116(1-3), pp. 194-201. Amsterdam: Elsevier 10.1016/j.vetmic.2006.03.003

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Strains of [Actinobacillus] rossii, [Pasteurella] mairii and [Pasteurella] aerogenes can be isolated from abortion in swine. The RTX toxin Pax has previously been found only in those [P.] aerogenes strains isolated from abortion. Nothing is known about RTX toxins in field isolates of the other two species. To gain insight into the distribution of selected RTX toxin genes and their association with abortion, PCR screening for the pax, apxII and apxIII operons on 21 [A.] rossii and seven [P.] mairii isolates was done. Since species can be phenotypically misidentified, the study was backed up by a phylogenetic analysis of all strains based on 16S rRNA, rpoB and infB genes. The pax gene was detected in all [P.] mairii but not in [A.] rossii strains. No apx genes were found in [P.] mairii but different gene combinations for apx were detected in [A.] rossii strains. Most of these strains were positive for apxIII, either alone or in combination with apxII. Whereas pax was found to be associated to strains from abortion no such indication could be found with apx in [A.] rossii strains. Phylogenetically [A.] rossii strains formed a heterogeneous cluster separated from Actinobacillus sensu stricto. [P.] mairii strains clustered with [P.] aerogenes but forming a separate branch. The fact that [P.] aerogenes, [P.] mairii and [A.] rossii can phylogenetically clearly be identified and might contain distinct RTX toxin genes allows their proper diagnosis and will further help to investigate their role as pathogens.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Korczak, Bozena; Frey, Joachim and Kuhnert, Peter

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

06 Dec 2014 03:01

Publisher DOI:

10.1016/j.vetmic.2006.03.003

PubMed ID:

16621343

Web of Science ID:

000239855000022

BORIS DOI:

10.7892/boris.18655

URI:

https://boris.unibe.ch/id/eprint/18655 (FactScience: 860)

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