Vertical transmission of a fluoroquinolone-resistant Escherichia coli within an integrated broiler operation

Petersen, Andreas; Christensen, Jens P; Kuhnert, Peter; Bisgaard, Magne; Olsen, John E (2006). Vertical transmission of a fluoroquinolone-resistant Escherichia coli within an integrated broiler operation. Veterinary microbiology, 116(1-3), pp. 120-128. Amsterdam: Elsevier 10.1016/j.vetmic.2006.03.015

[img] Text
1-s2.0-S0378113506001258-main.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (180kB) | Request a copy

The epidemiology of an enrofloxacin-resistant Escherichia coli clone was investigated during two separate outbreaks of colibacillosis in the Danish broiler production. In total five flocks were reported affected by the outbreaks. Recorded first-week mortalities were in the range of 1.7-12.7%. The clone was first isolated from dead broilers and subsequently demonstrated in samples from associated hatchers and the parent flock with its embryonated eggs, suggesting a vertical transmission from the parents. The second outbreak involved two broiler flocks unrelated to the affected flocks from the first outbreak. However, the clone could not be demonstrated in the associated parent flock. Furthermore, samplings from grand-parent flocks were negative for the outbreak clone. The clonality was evaluated by plasmid profiling and pulsed-field gel electrophoresis. None of the recognized virulence factors were demonstrated in the outbreak clone by microarray and PCR assay. The molecular background for the fluoroquinolone-resistance was investigated and point mutations in gyrA and parC leading to amino-acid substitutions in quinolone-resistance determining regions of GyrA and ParC were demonstrated. Vertical transmission of enrofloxacin-resistant E. coli from healthy parents resulting in high first-week mortality in the offspring illustrates the potential of the emergence and spreading of fluoroquinolone-resistant bacteria in animal husbandry, even though the use of fluoroquinolones is restricted.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Kuhnert, Peter

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

06 Dec 2014 18:01

Publisher DOI:

10.1016/j.vetmic.2006.03.015

PubMed ID:

16650662

Web of Science ID:

000239855000014

BORIS DOI:

10.7892/boris.18658

URI:

https://boris.unibe.ch/id/eprint/18658 (FactScience: 864)

Actions (login required)

Edit item Edit item
Provide Feedback