Expression of enterotoxigenic Escherichia coli colonization factors in Vibrio cholerae

Favre, Didier; Lüdi, Stefan; Stoffel, Michael Hubert; Frey, Joachim; Horn, Michael P; Dietrich, Guido; Spreng, Simone; Viret, Jean-François (2006). Expression of enterotoxigenic Escherichia coli colonization factors in Vibrio cholerae. Vaccine, 24(20), pp. 4354-68. Amsterdam: Elsevier 10.1016/j.vaccine.2006.02.052

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As a first step towards a vaccine against diarrhoeal disease caused by enterotoxigenic Escherichia coli (ETEC), we have studied the expression of several ETEC antigens in the live attenuated Vibrio cholerae vaccine strain CVD 103-HgR. Colonization factors (CF) CFA/I, CS3, and CS6 were expressed at the surface of V. cholerae CVD 103-HgR. Both CFA/I and CS3 required the co-expression of a positive regulator for expression, while CS6 was expressed without regulation. Up-regulation of CF expression in V. cholerae was very efficient, so that high amounts of CFA/I and CS3 similar to those in wild-type ETEC were synthesized from chromosomally integrated CF and positive regulator loci. Increasing either the operon and/or the positive regulator gene dosage resulted in only a small increase in CFA/I and CS3 expression. In contrast, the level of expression of the non-regulated CS6 fimbriae appeared to be more dependent on gene dosage. While CF expression in wild-type ETEC is known to be tightly thermoregulated and medium dependent, it seems to be less stringent in V. cholerae. Finally, co-expression of two or three CFs in the same strain was efficient even under the control of one single regulator gene.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Stoffel, Michael Hubert and Frey, Joachim

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0264-410X

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

21 Jan 2014 15:05

Publisher DOI:

10.1016/j.vaccine.2006.02.052

PubMed ID:

16581160

Web of Science ID:

000238016600015

URI:

https://boris.unibe.ch/id/eprint/18662 (FactScience: 869)

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