PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections

Schild, Marc; Gianinazzi, Christian; Gottstein, Bruno; Müller, Norbert (2007). PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections. Journal of clinical microbiology, 45(2), pp. 564-7. Washington, D.C.: American Society for Microbiology 10.1128/JCM.01357-06

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We developed a real-time PCR which allowed the highly sensitive detection of Naegleria fowleri in histological brain tissue sections from experimentally infected mice. This genus-specific small-subunit (18S) rRNA gene-based PCR can complement conventional (immuno-) histology for the diagnosis of primary amoebic meningoencephalitis in paraffin-embedded brain necropsy specimens that had been fixed in formalin buffered with phosphate-buffered saline.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Schild, Marc; Gianinazzi, Christian; Gottstein, Bruno and Müller, Norbert

ISSN:

0095-1137

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

04 May 2014 23:13

Publisher DOI:

10.1128/JCM.01357-06

PubMed ID:

17121998

Web of Science ID:

000244270000049

URI:

https://boris.unibe.ch/id/eprint/18665 (FactScience: 872)

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