Proteomic analysis of stimulated saliva in gastroesophageal reflux disease patients with and without erosive tooth wear: observational study.

Martini, Tatiana; Câmara, João Victor Frazão; Dionizio, Aline; Ventura, Talita Mendes Oliveira; Cassiano, Luiza de Paula Silva; de Souza E Silva, Cíntia Maria; Taira, Even Akemi; Araujo, Tamara Teodoro; Santos, Lethycia Almeida; Ferrari, Carolina Ruis; Magalhães, Ana Carolina; Saads Carvalho, Thiago; Baumann, Tommy; Lussi, Adrian; Rios, Daniela; de Oliveira, Ricardo Brandt; Palma-Dibb, Regina Guenka; Buzalaf, Marília Afonso Rabelo (2023). Proteomic analysis of stimulated saliva in gastroesophageal reflux disease patients with and without erosive tooth wear: observational study. Journal of dentistry, 139, p. 104724. Elsevier Science 10.1016/j.jdent.2023.104724

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To evaluate the difference in the proteomic profile of stimulated saliva in patients with gastroesophageal reflux disease (GERD) with (GE) and without (GNE) erosive tooth wear (ETW), regarding both human and bacterial proteins.


Stimulated saliva (SS) was collected from 16 patients (8/group). Samples were centrifuged at 4.500g for 15 min under refrigeration to remove all debris. The supernatant from each saliva sample was taken and frozen at -80°C. After extracting the proteins, they were submitted to reverse phase liquid chromatography and mass spectrometry (nLC-ESI-MS/MS). Label-free proteomic quantification was performed using Protein Lynx Global Service (PLGS) software (p < 0.05) for human and bacterial proteins.


In total, 67 human proteins were common for GNE and GE groups. GNE group presented, compared to GE group, increase in proteins that confer antimicrobial and acid resistant properties, such as cystatins, histatin and immunoglobulins. However, GNE group had a marked decrease in subunits of hemoglobin (α, β and delta). Regarding bacterial proteins, for SS, 7 and 10 unique proteins were identified in the GE and GNE groups, respectively. They are related to protein synthesis and energy metabolism and interact with human proteins typically found in saliva and supramolecular complexes of the acquired pellicle.


Our data indicate that the stimulation of the salivary flow increases acid resistant and antimicrobial proteins in saliva, which might protect against ETW.


This pioneer study showed important differences in the human and bacterial proteome of SS in patients with GERD with or without ETW.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry
04 Faculty of Medicine > School of Dental Medicine

UniBE Contributor:

Saads Carvalho, Thiago, Baumann, Tommy, Lussi, Adrian


600 Technology > 610 Medicine & health




Elsevier Science




Pubmed Import

Date Deposited:

09 Oct 2023 10:49

Last Modified:

26 Nov 2023 00:16

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Gastroesophageal Reflux Proteomics Saliva Tooth Erosion




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