Parisi, Ludovica; Bianchi, Massimiliano Giovanni; Ghezzi, Benedetta; Maurizi, Eleonora; Macaluso, Guido Maria; Bussolati, Ovidio; Lumetti, Simone (2023). Preparation of human primary macrophages to study the polarization from monocyte-derived macrophages to pro- or anti-inflammatory macrophages at biomaterial interface in vitro. Journal of dental sciences, 18(4), pp. 1630-1637. Elsevier 10.1016/j.jds.2023.01.020
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BACKGROUND/PURPOSE
Testing of dental materials when in contact with innate immune cells has been so far hindered by the lack of proper in vitro models. Human primary monocyte-derived macrophages (MDMs) would be an excellent option to this aim. However, the inability to detach them from the tissue culture plates contrast the possibility to culture them on biomaterials. The goal of the present work is to present and validate an innovative protocol to obtain MDMs from peripheral blood monocytes, and to reseed them in contact with biomaterials without altering their viability and phenotype.
MATERIALS AND METHODS
We differentiated MDMs on ultra-low attachment tissue culture plastics and recovered them with specific detachment solution in order to be reseeded on a secondary substrate. Therefore, using biological assays (RT-PCR, Western blot, and immunofluorescence) we compared their phenotype to MDMs differentiated on standard culture plates.
RESULTS
Transferred MDMs keep their differentiated M0 resting state, as well as the ability to be polarized into M1 (pro-inflammatory) or M2 (anti-inflammatory) macrophages.
CONCLUSION
These data provide the dental material research community the unprecedented possibility to investigate the immunomodulatory properties of biomaterials for dental application.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics |
UniBE Contributor: |
Parisi, Ludovica |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2213-8862 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
09 Oct 2023 10:09 |
Last Modified: |
09 Oct 2023 10:20 |
Publisher DOI: |
10.1016/j.jds.2023.01.020 |
PubMed ID: |
37799917 |
Uncontrolled Keywords: |
Biocompatible materials Bone substitutes Dental implants Human Macrophage inflammation Macrophages |
BORIS DOI: |
10.48350/186956 |
URI: |
https://boris.unibe.ch/id/eprint/186956 |
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