Aromatase in zebrafish: a potential target for endocrine disrupting chemicals

Kallivretaki, Evangelina; Eggen, Rik; Neuhauss, Stephan; Alberti, Martin; Kausch, Ulf; Segner, Helmut (2006). Aromatase in zebrafish: a potential target for endocrine disrupting chemicals. Marine environmental research, 62 Suppl, S187-S190. London: Applied Science Publ. Ltd. 10.1016/j.marenvres.2006.04.003

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In zebrafish, two isoforms of the aromatase gene exist, namely cyp19a1 and cyp19a2, expressed predominantly in the gonads and brain, respectively. In this study, we focus on characterizing the specificity of antibodies against the aromatase isoforms, and on (xeno)estrogen-induced changes of individual cyp19a2 mRNA concentrations in the brains of adult male zebrafish. Among three polyclonal antibodies studied, the one against CYP19A2 was found to be specific in Western blots and immunohistochemistry. Real-time RT-PCR analyses revealed strong interindividual variation of cyp19a2 levels in the brains of adult male zebrafish. After a three-week-exposure to (xeno)estrogens, mean values of cyp19a2 mRNA levels tended to increase, with significant induction at 200 ng 17beta-estradiol/L, but interindividual variation of cyp19a2 expression was maintained.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Kallivretaki, Evangelina and Segner, Helmut

Subjects:

600 Technology > 630 Agriculture

ISSN:

0141-1136

Publisher:

Applied Science Publ. Ltd.

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

08 Jun 2016 10:38

Publisher DOI:

10.1016/j.marenvres.2006.04.003

PubMed ID:

16698076

Web of Science ID:

000238957800140

URI:

https://boris.unibe.ch/id/eprint/18701 (FactScience: 911)

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