Dawson, K L D; Wildi, N; Koch, M C; Oevermann, A; Rosato, G; Grest, P; Hilbe, M; Seuberlich, T (2023). Virus discovery in dogs with non-suppurative encephalitis reveals a high incidence of tick-borne encephalitis virus infections in Switzerland. Schweizer Archiv für Tierheilkunde, 165(10), pp. 656-666. Gesellschaft Schweizer Tierärztinnen und Tierärzte 10.17236/sat00407
Full text not available from this repository. (Request a copy)Viral infections are a frequent cause of disseminated non-suppurative encephalitis in dogs. However, using routine diagnostic methods, the specific virus may remain unknown due to extensive or complete viral clearance or because the virus is unexpected or new. A metatranscriptomics-based approach of combining high-throughput sequencing (HTS) and bioinformatics analysis was used to investigate the viral etiology in archival cases of dogs with non-suppurative encephalitis. In formalin-fixed paraffin embedded (FFPE) brain material from the years 1976 to 2021 a high incidence of tick-borne encephalitis virus (TBEV) was detected. Moreover, canine distemper virus (CDV) was identified without typical demyelinating lesions and canine vesivirus (CaVV) was detected as an unexpected virus associated with non-suppurative encephalitis. We demonstrated the viral presence in brain tissues at the sites of inflammation by immunohistochemistry (IHC) and in situ hybridization (ISH). These results highlight the value of emerging sequencing technologies in veterinary diagnostics and expand our knowledge on the etiologies of encephalitis in dogs.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) |
UniBE Contributor: |
Dawson, Kara Lynne Dianne, Wildi, Nicole, Koch, Michel Christoph, Oevermann, Anna, Seuberlich, Torsten |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
1664-2848 |
Publisher: |
Gesellschaft Schweizer Tierärztinnen und Tierärzte |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
18 Oct 2023 12:32 |
Last Modified: |
18 Oct 2023 12:32 |
Publisher DOI: |
10.17236/sat00407 |
PubMed ID: |
37822248 |
Uncontrolled Keywords: |
Cani Chiens Enzephalitis Hochdurchsatzsequenzierung Hunde Metatranskriptomik Virus dogs encefalite encephalitis encéphalite high-throughput sequencing metatranscriptomica metatranscriptomics métatranscriptomique sequenziamento ad alta capacità séquençage à haut débit virus |
URI: |
https://boris.unibe.ch/id/eprint/187139 |
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