Liu, Huan; Ishikawa-Ankerhold, Hellen; Winterhalter, Julia; Lorenz, Michael; Vladymyrov, Mykhailo; Massberg, Steffen; Schulz, Christian; Orban, Mathias (2023). Multiphoton In Vivo Microscopy of Embryonic Thrombopoiesis Reveals the Generation of Platelets through Budding. Cells, 12(19) MDPI 10.3390/cells12192411
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Platelets are generated by specialized cells called megakaryocytes (MKs). However, MK's origin and platelet release mode have remained incompletely understood. Here, we established direct visualization of embryonic thrombopoiesis in vivo by combining multiphoton intravital microscopy (MP-IVM) with a fluorescence switch reporter mouse model under control of the platelet factor 4 promoter (Pf4CreRosa26mTmG). Using this microscopy tool, we discovered that fetal liver MKs provide higher thrombopoietic activity than yolk sac MKs. Mechanistically, fetal platelets were released from MKs either by membrane buds or the formation of proplatelets, with the former constituting the key process. In E14.5 c-Myb-deficient embryos that lack definitive hematopoiesis, MK and platelet numbers were similar to wild-type embryos, indicating the independence of embryonic thrombopoiesis from definitive hematopoiesis at this stage of development. In summary, our novel MP-IVM protocol allows the characterization of thrombopoiesis with high spatio-temporal resolution in the mouse embryo and has identified membrane budding as the main mechanism of fetal platelet production.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Physics Institute > Laboratory for High Energy Physics (LHEP) 04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute 10 Strategic Research Centers > Albert Einstein Center for Fundamental Physics (AEC) |
UniBE Contributor: |
Vladymyrov, Mykhailo |
Subjects: |
500 Science > 530 Physics 600 Technology > 610 Medicine & health |
ISSN: |
2073-4409 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
16 Oct 2023 13:35 |
Last Modified: |
17 Oct 2023 09:59 |
Publisher DOI: |
10.3390/cells12192411 |
PubMed ID: |
37830625 |
Uncontrolled Keywords: |
c-Myb fetal liver megakaryocyte multiphoton intravital microscopy thrombopoiesis yolk sac |
BORIS DOI: |
10.48350/187178 |
URI: |
https://boris.unibe.ch/id/eprint/187178 |
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