Use and relevance of a bovine mammary gland explant model to study infection responses in bovine mammary tissue

Rabot, Aline; Wellnitz, Olga; Meyer, Heinrich H D; Bruckmaier, Rupert M (2007). Use and relevance of a bovine mammary gland explant model to study infection responses in bovine mammary tissue. Journal of dairy research, 74(1), pp. 93-9. London: Cambridge University Press 10.1017/S0022029906002147

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Our aim was to develop an explant model to define more precisely the early response of bovine mammary epithelial cells to infection. Therefore we investigated the mRNA expression encoding for some soluble immunological factors in lipopolysaccharide (LPS)-treated bovine mammary gland explants. Explants were taken out from the mammary gland of eight lactating cows after slaughter then incubated with LPS (10 mug/ml) for 6 h. The mRNA expression of alpha-lactalbumin (alpha-la), various cytokines, tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, IL-8, and two immunoglobulin receptors, the neonatal Fc receptor (FcRn) and polymeric immunoglobulin receptor (pIGR), were assessed with qPCR before and after 3 h and 6 h of LPS challenge. Both immunoglobulin receptors and alpha-la increased at 3 h then recovered their initial level at 6 h whereas IL-1beta, IL-6 and IL-8 increased only after 6 h (P<0.05). Surprisingly, TNF-alpha transcripts did not show any regulation in response to the LPS treatment. We nevertheless concluded that our model was valid to examine the short-term response of mammary epithelial cell challenged with LPS.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology

UniBE Contributor:

Wellnitz, Olga and Bruckmaier, Rupert

ISSN:

0022-0299

Publisher:

Cambridge University Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

04 May 2014 23:13

Publisher DOI:

10.1017/S0022029906002147

PubMed ID:

16978438

Web of Science ID:

000245024800014

URI:

https://boris.unibe.ch/id/eprint/18749 (FactScience: 987)

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