Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs

Forterre, Simone; Raila, Jens; Forterre, Franck; Brunnberg, Leo; Schweigert, Florian J (2006). Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs. Veterinary journal, 171(3), pp. 451-5. Amsterdam: Elsevier 10.1016/j.tvjl.2004.11.017

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The aim of this study was to investigate differences in concentrations of vitamin A, transthyretin (TTR) and retinol-binding protein (RBP) between plasma and cerebrospinal fluid (CSF) in dogs. RBP was detected using ELISA, and both RBP and TTR by Western blot analysis after separation on SDS-PAGE. Vitamin A was determined by high performance liquid chromatography. RBP and TTR as well as vitamin A were detected in all samples but at substantially lower concentrations in CSF compared to plasma. RBP in dog plasma showed a similar molecular mass to that of humans, whereas canine TTR had a lower molecular mass. Comparison between plasma and CSF showed that both RBP and TTR were of lower molecular mass in CSF. In CSF, RBP and retinol were present at 10-100-fold lower concentrations compared to plasma. Retinyl esters were present only in minute amounts in 5/17 samples. In conclusion, the CSF of dogs compared to humans is significantly different in terms of both quality and quantity of transport proteins for vitamin A.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)

UniBE Contributor:

Forterre, Simone and Forterre, Franck

Subjects:

600 Technology > 630 Agriculture

ISSN:

1090-0233

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:45

Last Modified:

17 Sep 2014 10:43

Publisher DOI:

10.1016/j.tvjl.2004.11.017

PubMed ID:

16624711

Web of Science ID:

000237560600011

URI:

https://boris.unibe.ch/id/eprint/18841 (FactScience: 1087)

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