Caveolin-1 is required for signaling and membrane targeting of EphB1 receptor tyrosine kinase

Vihanto, Meri M; Vindis, Cecile; Djonov, Valentin; Cerretti, Douglas P; Huynh-Do, Uyen (2006). Caveolin-1 is required for signaling and membrane targeting of EphB1 receptor tyrosine kinase. Journal of cell science, 119(Pt 11), pp. 2299-309. Cambridge: Company of Biologists Limited 10.1242/jcs.02946

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Eph receptor tyrosine kinases are key players during the development of the embryonic vasculature; however, their role and regulation in adult angiogenesis remain to be defined. Caveolae are flask-shaped invaginations of the cell membrane; their major structural protein, caveolin-1, has been shown to regulate signaling molecules localized in these micro-domains. The interaction of caveolin-1 with several of these proteins is mediated by the binding of its scaffolding domain to a region containing hydrophobic amino acids within these proteins. The presence of such a motif within the EphB1 kinase domain prompted us to investigate the caveolar localization and regulation of EphB1 by caveolin-1. We report that EphB1 receptors are localized in caveolae, and directly interact with caveolin-1 upon ligand stimulation. This interaction, as well as EphB1-mediated activation of extracellular-signal-regulated kinase (ERK), was abrogated by overexpression of a caveolin-1 mutant lacking a functional scaffolding domain. Interaction between Ephs and caveolin-1 is not restricted to the B-subclass of receptors, since we show that EphA2 also interacts with caveolin-1. Furthermore, we demonstrate that the caveolin-binding motif within the kinase domain of EphB1 is primordial for its correct membrane targeting. Taken together, our findings establish caveolin-1 as an important regulator of downstream signaling and membrane targeting of EphB1.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension

UniBE Contributor:

Djonov, Valentin Georgiev and Huynh-Do, Uyen


600 Technology > 610 Medicine & health






Company of Biologists Limited




Factscience Import

Date Deposited:

04 Oct 2013 14:46

Last Modified:

31 Dec 2020 08:06

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URI: (FactScience: 1208)

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