A new microbiopsy system enables rapid preparation of tissue for high-pressure freezing

Vanhecke, Dimitri; Eggli, Peter; Graber, Werner; Studer, Daniel (2006). A new microbiopsy system enables rapid preparation of tissue for high-pressure freezing. Methods in molecular biology, 319, pp. 463-477. Totowa, N.J.: Humana Press

Full text not available from this repository. (Request a copy)

A microbiopsy system was developed to overcome long sampling times for tissues before they are cryo-fixed by high-pressure freezing. A commercially available biopsy gun was adapted to the needs of small-organ excisions, and biopsy needles were modified to allow small samples (0.6 mm x 1.2 mm x 0.3 mm) to be taken. Specimen platelets with a central slot of the same dimensions as the biopsy are used. A self-made transfer device (in the meantime optimized by Leica-Microsystems [Vienna, Austria]) coordinates the transfer of the excised sample from the biopsy needle into the platelet slot and the subsequent loading in a specimen holder, which is then introduced into a high-pressure freezer (Leica EM PACT; Leica Microsystems, Vienna, Austria). Thirty seconds preparation time is needed from excision until high-pressure freezing. Brain, liver, kidney and muscle excisions of anesthetised rats are shown to be well frozen.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Eggli, Peter and Studer, Daniel Franz

ISSN:

1064-3745

ISBN:

16719368

Publisher:

Humana Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:46

Last Modified:

08 Jun 2016 10:39

PubMed ID:

16719368

URI:

https://boris.unibe.ch/id/eprint/18939 (FactScience: 1210)

Actions (login required)

Edit item Edit item
Provide Feedback