Panahipour, Layla; Abbasabadi, Azarakhsh Oladzad; Wagner, Anja; Kratochwill, Klaus; Pichler, Monika; Gruber, Reinhard (2023). Bone Allograft Acid Lysates Change the Genetic Signature of Gingival Fibroblasts. International journal of molecular sciences, 24(22) MDPI 10.3390/ijms242216181
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Bone allografts are widely used as osteoconductive support to guide bone regrowth. Bone allografts are more than a scaffold for the immigrating cells as they maintain some bioactivity of the original bone matrix. Yet, it remains unclear how immigrating cells respond to bone allografts. To this end, we have evaluated the response of mesenchymal cells exposed to acid lysates of bone allografts (ALBA). RNAseq revealed that ALBA has a strong impact on the genetic signature of gingival fibroblasts, indicated by the increased expression of IL11, AREG, C11orf96, STC1, and GK-as confirmed by RT-PCR, and for IL11 and STC1 by immunoassays. Considering that transforming growth factor-β (TGF-β) is stored in the bone matrix and may have caused the expression changes, we performed a proteomics analysis, TGF-β immunoassay, and smad2/3 nuclear translocation. ALBA neither showed detectable TGF-β nor was the lysate able to induce smad2/3 translocation. Nevertheless, the TGF-β receptor type I kinase inhibitor SB431542 significantly decreased the expression of IL11, AREG, and C11orf96, suggesting that other agonists than TGF-β are responsible for the robust cell response. The findings suggest that IL11, AREG, and C11orf96 expression in mesenchymal cells can serve as a bioassay reflecting the bioactivity of the bone allografts.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology |
UniBE Contributor: |
Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1422-0067 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
28 Nov 2023 15:07 |
Last Modified: |
28 Nov 2023 15:16 |
Publisher DOI: |
10.3390/ijms242216181 |
PubMed ID: |
38003371 |
Uncontrolled Keywords: |
AREG C11orf96 IL11 RNAseq bone allograft bone augmentation bone regeneration gingival fibroblasts |
BORIS DOI: |
10.48350/189418 |
URI: |
https://boris.unibe.ch/id/eprint/189418 |
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