Eggers, Benedikt; Stope, Matthias Bernhard; Marciniak, Jana; Mustea, Alexander; Eick, Sigrun; Deschner, James; Nokhbehsaim, Marjan; Kramer, Franz-Josef (2023). Non-Invasive Physical Plasma Reduces the Inflammatory Response in Microbially Prestimulated Human Gingival Fibroblasts. International journal of molecular sciences, 24(22) MDPI 10.3390/ijms242216156
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Non-invasive physical plasma (NIPP), an electrically conductive gas, is playing an increasingly important role in medicine due to its antimicrobial and regenerative properties. However, NIPP is not yet well established in dentistry, although it has promising potential, especially for periodontological applications. The aim of the present study was to investigate the effect of NIPP on a commercially available human gingival fibroblast (HGF) cell line and primary HGFs in the presence of periodontitis-associated bacteria. First, primary HGFs from eight patients were characterised by immunofluorescence, and cell numbers were examined by an automatic cell counter over 5 days. Then, HGFs that were preincubated with Fusobacterium nucleatum (F.n.) were treated with NIPP. Afterwards, the IL-6 and IL-8 levels in the cell supernatants were determined by ELISA. In HGFs, F.n. caused a significant increase in IL-6 and IL-8, and this F.n.-induced upregulation of both cytokines was counteracted by NIPP, suggesting a beneficial effect of physical plasma on periodontal cells in a microbial environment. The application of NIPP in periodontal therapy could therefore represent a novel and promising strategy and deserves further investigation.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology |
UniBE Contributor: |
Eick, Sigrun |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1422-0067 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
28 Nov 2023 15:04 |
Last Modified: |
28 Nov 2023 15:13 |
Publisher DOI: |
10.3390/ijms242216156 |
PubMed ID: |
38003346 |
Uncontrolled Keywords: |
antimicrobial effect cold plasma dentistry gingival fibroblasts inflammation non-invasive physical plasma non-thermal plasma periodontology |
BORIS DOI: |
10.48350/189419 |
URI: |
https://boris.unibe.ch/id/eprint/189419 |
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