Wang, Lei; Erb, Matthias (2024). Feeding Assay to Study the Effect of Phytocytokines on Direct and Indirect Defense in Maize. Methods in molecular biology, 2731, pp. 133-142. Springer 10.1007/978-1-0716-3511-7_10
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Phytocytokines mediate defense against pests and pathogens. Many methods have been developed to study the physiological responses triggered by phytocytokines in dicot plants. Here, we describe a detailed peptide feeding protocol to study the effect of phytocytokines on direct and indirect anti-herbivore defense in maize. This method relies on peptide uptake by the excised maize seedling or leaves via the transpiration stream. The headspace volatiles from plant samples are then analyzed by proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS), or by gas chromatography-mass spectrometry (GC-MS). The samples can also be further processed to evaluate phytocytokine-induced defense gene expression or phytohormone production.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Biotic Interactions 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) |
UniBE Contributor: |
Wang, Lei (B), Erb, Matthias |
Subjects: |
500 Science > 580 Plants (Botany) 500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1940-6029 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
04 Dec 2023 10:45 |
Last Modified: |
25 Mar 2024 09:24 |
Publisher DOI: |
10.1007/978-1-0716-3511-7_10 |
PubMed ID: |
38019431 |
Uncontrolled Keywords: |
Anti-herbivore defense Indirect defense Maize Peptide feeding Phytocytokine Plant volatile Plant-insect interactions Proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) |
BORIS DOI: |
10.48350/189637 |
URI: |
https://boris.unibe.ch/id/eprint/189637 |
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