Ruchti, Nadine; Braissant, Olivier; Overesch, Gudrun (2024). Real time detection of pathogenic bacteria in veterinary microbiology using isothermal microcalorimetry - A different approach. Veterinary microbiology, 288(109929), p. 109929. Elsevier 10.1016/j.vetmic.2023.109929
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With today's challenges regarding antibiotic resistance and the importance of the implementation of prudent use of antibiotics, fast and reliable diagnostic tools for bacterial infections and subsequent antimicrobial susceptibility testing are of utmost relevance. Isothermal microcalorimetry (IMC) is a broadly applicable method, with which metabolic heat flow in reproducing bacteria can be measured in real time. To the best of the authors' knowledge, this is the first report on examination of 124 urine samples from feline and canine urinary tract infection with an IMC-based prototype instrument. A concentration-dependent time of peak heat flow by dilution series with Escherichia coli and Enterococcus faecalis reference strains demonstrated the general good performance of the prototype for detection of these bacteria. With diagnostic culture being set as a gold standard, the diagnostic sensitivity of IMC compared to bacteriological culture was 80 %, the diagnostic specificity was 97 %. With a Cohens' kappa value (κ) of 0.80, the two methods show good concordance. The results from our study demonstrate that the IMC technology is suitable to allow reliable, but much faster detection of bacteria than conventional culture, especially for Escherichia coli. Thus, implementing IMC technology could markedly speed up the bacteriological diagnostic process in veterinary medicine.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology |
UniBE Contributor: |
Ruchti, Nadine Julia, Overesch, Gudrun |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0378-1135 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
06 Dec 2023 15:34 |
Last Modified: |
30 Dec 2023 00:16 |
Publisher DOI: |
10.1016/j.vetmic.2023.109929 |
PubMed ID: |
38048670 |
Uncontrolled Keywords: |
Bacterial cystitis Bacteriological culture Calorimeter Cats Dogs Metabolic heat Urinary tract infection |
BORIS DOI: |
10.48350/189835 |
URI: |
https://boris.unibe.ch/id/eprint/189835 |
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