Structural maintenance of chromosomes (SMC) proteins are required for DNA elimination in Paramecium.

Zhang, Fukai; Bechara, Sebastian; Nowacki, Mariusz (2024). Structural maintenance of chromosomes (SMC) proteins are required for DNA elimination in Paramecium. Life science alliance, 7(2) EMBO Press 10.26508/lsa.202302281

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Chromosome (SMC) proteins are a large family of ATPases that play important roles in the organization and dynamics of chromatin. They are central regulators of chromosome dynamics and the core component of condensin. DNA elimination during zygotic somatic genome development is a characteristic feature of ciliated protozoa such as Paramecium This process occurs after meiosis, mitosis, karyogamy, and another mitosis, which result in the formation of a new germline and somatic nuclei. The series of nuclear divisions implies an important role of SMC proteins in Paramecium sexual development. The relationship between DNA elimination and SMC has not yet been described. Here, we applied RNA interference, genome sequencing, mRNA sequencing, immunofluorescence, and mass spectrometry to investigate the roles of SMC components in DNA elimination. Our results show that SMC4-2 is required for genome rearrangement, whereas SMC4-1 is not. Functional diversification of SMC4 in Paramecium led to a formation of two paralogues where SMC4-2 acquired a novel, development-specific function and differs from SMC4-1. Moreover, our study suggests a competitive relationship between these two proteins.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Zhang, Fukai, Bechara, Sebastian Toni, Nowacki, Mariusz

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2575-1077

Publisher:

EMBO Press

Language:

English

Submitter:

Pubmed Import

Date Deposited:

11 Dec 2023 12:13

Last Modified:

12 Dec 2023 16:25

Publisher DOI:

10.26508/lsa.202302281

PubMed ID:

38056908

BORIS DOI:

10.48350/189913

URI:

https://boris.unibe.ch/id/eprint/189913

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