Zhang, Fukai; Bechara, Sebastian; Nowacki, Mariusz (2024). Structural maintenance of chromosomes (SMC) proteins are required for DNA elimination in Paramecium. Life science alliance, 7(2) EMBO Press 10.26508/lsa.202302281
|
Text
e202302281.full.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (3MB) | Preview |
Chromosome (SMC) proteins are a large family of ATPases that play important roles in the organization and dynamics of chromatin. They are central regulators of chromosome dynamics and the core component of condensin. DNA elimination during zygotic somatic genome development is a characteristic feature of ciliated protozoa such as Paramecium This process occurs after meiosis, mitosis, karyogamy, and another mitosis, which result in the formation of a new germline and somatic nuclei. The series of nuclear divisions implies an important role of SMC proteins in Paramecium sexual development. The relationship between DNA elimination and SMC has not yet been described. Here, we applied RNA interference, genome sequencing, mRNA sequencing, immunofluorescence, and mass spectrometry to investigate the roles of SMC components in DNA elimination. Our results show that SMC4-2 is required for genome rearrangement, whereas SMC4-1 is not. Functional diversification of SMC4 in Paramecium led to a formation of two paralogues where SMC4-2 acquired a novel, development-specific function and differs from SMC4-1. Moreover, our study suggests a competitive relationship between these two proteins.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology |
UniBE Contributor: |
Zhang, Fukai, Bechara, Sebastian Toni, Nowacki, Mariusz |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
2575-1077 |
Publisher: |
EMBO Press |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
11 Dec 2023 12:13 |
Last Modified: |
12 Dec 2023 16:25 |
Publisher DOI: |
10.26508/lsa.202302281 |
PubMed ID: |
38056908 |
BORIS DOI: |
10.48350/189913 |
URI: |
https://boris.unibe.ch/id/eprint/189913 |
Actions (login required)
 |
Edit item |