Reflecting the human lip in vitro: Cleft lip skin and mucosa keratinocytes keep their identities.

Rihs, Silvia; Parisi, Ludovica; Lauener, Anic; Mansour, Farah; Schnyder, Isabelle; Dekany, Gabriela M; La Scala, Giorgio C; Katsaros, Christos; Degen, Martin (2024). Reflecting the human lip in vitro: Cleft lip skin and mucosa keratinocytes keep their identities. (In Press). Oral diseases Wiley 10.1111/odi.14844

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OBJECTIVES

Cell models have shown great promise as tools for research, potentially providing intriguing alternatives to animal models. However, the original tissue characteristics must be maintained in culture, a fact that is often assumed, but seldom assessed. We aimed to follow the retention of the original tissue identities of cleft lip-derived skin and mucosa keratinocytes in vitro.

METHODS

Cleft lip-derived keratinocytes were isolated from discarded tissue along the cleft margins during cheiloplasty. Cell identities were assessed by immunohistochemistry and quantitative real-time PCR for tissue-specific markers and compared with native lip tissue. Moreover, keratinocytes were regularly analyzed for the retention of the original tissue characteristics by the aforementioned methods as well as by differentiation assays.

RESULTS

The various anatomical zones of the human lip could be distinguished using a panel of differentiation and functional-based markers. Using these markers, retention of the original tissue identities could be followed and confirmed in the corresponding primary keratinocytes in culture.

CONCLUSIONS

Our findings promote patient-derived cells retaining their original identities as astonishing and clinically relevant in vitro tools. Such cells allow a better molecular understanding of various lip-associated pathologies as well as their modeling in vitro, including but not restricted to orofacial clefts.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Orthodontic Research
04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Surgery

UniBE Contributor:

Rihs, Silvia, Parisi, Ludovica, Lauener, Anic Sara, Mansour, Farah, Schnyder, Isabelle, Dekany, Gabriela Marta, Katsaros, Christos, Degen, Martin

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1354-523X

Publisher:

Wiley

Language:

English

Submitter:

Pubmed Import

Date Deposited:

08 Jan 2024 10:50

Last Modified:

09 Jan 2024 15:49

Publisher DOI:

10.1111/odi.14844

PubMed ID:

38178623

Uncontrolled Keywords:

cell differentiation cleft lip keratinocytes mouth mucosa skin three-dimensional cell culture

BORIS DOI:

10.48350/191301

URI:

https://boris.unibe.ch/id/eprint/191301

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