Meirelles, Matheus A.; Reymond, Jean-Louis (2023). Optimizing Phosphate Couplings for Dolichyl Diphosphochitobiose to Enable Protein N ‐Glycosylation Studies. Helvetica chimica acta, 106(12) Wiley 10.1002/hlca.202300171
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Hereinwe reportan optimizedsynthesisfor dolichyldiphosphochitobiose(GlcNAc2-PP-Dol25), a probeusefulforbiochemicalandstructuralstudiesof proteinN-glycosylationin eukaryoticcells.Weimprovedthreephosphatecouplingstepsin termsof yieldsandreactiontimes,namelychitobiosephosphorylation,dolicholphosphorylation,andphosphate-phosphatecoupling,by adjustingreagentsandconditions.We alsodevelopedan efficientpreparativereverse-phaseHPLCpurificationprotocolfollowedby ion exchangestepto obtainthepureproductas a stablesodiumsalt.Theseoptimizedproceduresensurea reliablesupplyof GlcNAc2-PP-Dol25for enzymaticstudies.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Andrade Meirelles, Matheus, Reymond, Jean-Louis |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry 600 Technology > 610 Medicine & health |
ISSN: |
0018-019X |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Sandra Tanja Zbinden Di Biase |
Date Deposited: |
11 Jan 2024 10:13 |
Last Modified: |
11 Jan 2024 10:13 |
Publisher DOI: |
10.1002/hlca.202300171 |
BORIS DOI: |
10.48350/191468 |
URI: |
https://boris.unibe.ch/id/eprint/191468 |
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