McCloskey, Molly C; Kasap, Pelin; Trempel, Michelle; Widom, Louis P; Kuebel, Julia; Chen, Kaihua; Gaborski, Thomas R; Engelhardt, Britta; McGrath, James L (2024). Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier. Journal of visualized experiments(203) MYJoVE Corporation 10.3791/65258
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The microSiM (µSiM) is a membrane-based culture platform for modeling the blood-brain barrier (BBB). Unlike conventional membrane-based platforms, the µSiM provides experimentalists with new capabilities, including live cell imaging, unhindered paracrine signaling between 'blood' and 'brain' chambers, and the ability to directly image immunofluorescence without the need for the extraction/remounting of membranes. Here we demonstrate the basic use of the platform to establish monoculture (endothelial cells) and co-culture (endothelial cells and pericytes) models of the BBB using ultrathin nanoporous silicon-nitride membranes. We demonstrate compatibility with both primary cell cultures and human induced pluripotent stem cell (hiPSC) cultures. We provide methods for qualitative analysis of BBB models via immunofluorescence staining and demonstrate the use of the µSiM for the quantitative assessment of barrier function in a small molecule permeability assay. The methods provided should enable users to establish their barrier models on the platform, advancing the use of tissue chip technology for studying human tissues.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute |
UniBE Contributor: |
Kasap, Pelin, Engelhardt, Britta |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1940-087X |
Publisher: |
MYJoVE Corporation |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
30 Jan 2024 10:45 |
Last Modified: |
30 Jan 2024 10:54 |
Publisher DOI: |
10.3791/65258 |
PubMed ID: |
38284519 |
BORIS DOI: |
10.48350/192232 |
URI: |
https://boris.unibe.ch/id/eprint/192232 |
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