Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin.

Wei, Yan; Cheng, Yihong; Wei, Hongjiang; Wang, Yulan; Zhang, Xiaoxin; Miron, Richard J; Zhang, Yufeng; Qing, Shanglan (2024). Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin. Platelets, 35(2316745) Tayler & Francis 10.1080/09537104.2024.2316745

Text Development_of_a_super-hydrophilic_anaerobic_tube_for_the_optimization_of_platelet-rich_fibrin.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (12MB) | Request a copy

Horizontal platelet-rich fibrin (H-PRF) contains a variety of bioactive growth factors and cytokines that play a key role in the process of tissue healing and regeneration. The blood collection tubes used to produce Solid-PRF (plasmatrix (PM) tubes) have previously been shown to have a great impact on the morphology, strength and composition of the final H-PRF clot. Therefore, modification to PM tubes is an important step toward the future optimization of PRF. To this end, we innovatively modified the inner wall surface of the PM tubes with plasma and adjusted the gas environment inside the PM tubes to prepare super-hydrophilic anaerobic plasmatrix tubes (SHAP tubes). It was made anaerobic for the preparation of H-PRF with the aim of improving mechanical strength and bioactivity. The findings demonstrated that an anaerobic environment stimulated platelet activation within the PRF tubes. After compression, the prepared H-PRF membrane formed a fibrous cross-linked network with high fracture strength, ideal degradation characteristics, in addition to a significant increase in size. Thereafter, the H-PRF membranes prepared by the SHAP tubes significantly promoted collagen synthesis of gingival fibroblast and the mineralization of osteoblasts while maintaining excellent biocompatibility, and advantageous antibacterial properties. In conclusion, the newly modified PRF tubes had better platelet activation properties leading to better mechanical strength, a longer degradation period, and better regenerative properties in oral cell types including gingival fibroblast and alveolar osteoblasts. It also improves the success rate of H-PRF preparation in patients with coagulation dysfunction and expands the clinical application scenario.

Interest & Impact

Downloads

0 since deposited on 27 Feb 2024
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item