Fluorescent microangiography (FMA): an improved tool to visualize the pulmonary microvasculature

Dutly, Andre E; Kugathasan, Lakshmi; Trogadis, Judy E; Keshavjee, Shaf H; Stewart, Duncan J; Courtman, David W (2006). Fluorescent microangiography (FMA): an improved tool to visualize the pulmonary microvasculature. Laboratory investigation, 86(4), pp. 409-16. New York, N.Y.: Nature Publishing Group 10.1038/labinvest.3700399

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Visualization of the complex lung microvasculature and resolution of its three-dimensional architecture remains a difficult experimental challenge. We present a novel fluorescent microscopy technique to visualize both the normal and diseased pulmonary microvasculature. Physiologically relevant pulmonary perfusion conditions were applied using a low-viscosity perfusate infused under continuous airway ventilation. Intensely fluorescent polystyrene microspheres, confined to the vascular space, were imaged through confocal optical sectioning of 200 microm-thick lung sections. We applied this technique to rat lungs and the markedly enhanced depth of field in projected images allowed us to follow vascular branching patterns in both normal lungs and lungs from animals with experimentally induced pulmonary arterial hypertension. In addition, this method allowed complementary immunostaining and identification of cellular components surrounding the blood vessels. Fluorescent microangiography is a widely applicable and quantitative tool for the study of vascular changes in animal models of pulmonary disease.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery

UniBE Contributor:

Dutly, André Emanuel

ISSN:

0023-6837

ISBN:

16518405

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:47

Last Modified:

17 Mar 2015 21:46

Publisher DOI:

10.1038/labinvest.3700399

PubMed ID:

16518405

Web of Science ID:

000236665900009

URI:

https://boris.unibe.ch/id/eprint/19335 (FactScience: 1871)

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